The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding.

Shi Q, Chen Y, Li X, Dong H, Chen C, Zhong Z, Yang C, Liu G, Su D, Environ Microbiol 24(7):2994-3012 (2022) Europe PMC

SASDLC9 – 2-aminomuconic 6-semialdehyde dehydrogenase double truncation mutant (Δ124-138; Δ477-491)

2-aminomuconic 6-semialdehyde dehydrogenase
MWexperimental 50 kDa
MWexpected 101 kDa
VPorod 117 nm3
log I(s) 1.20×102 1.20×101 1.20×100 1.20×10-1
2-aminomuconic 6-semialdehyde dehydrogenase small angle scattering data  s, nm-1
ln I(s)
2-aminomuconic 6-semialdehyde dehydrogenase Guinier plot ln 1.21×102 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
2-aminomuconic 6-semialdehyde dehydrogenase Kratky plot 1.104 0 √3 sRg
p(r)
2-aminomuconic 6-semialdehyde dehydrogenase pair distance distribution function Rg: 2.8 nm 0 Dmax: 9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
2-aminomuconic 6-semialdehyde dehydrogenase PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of 2-aminomuconic 6-semialdehyde dehydrogenase double truncation mutant (Δ124-138; Δ477-491) in 20 mM HEPES, 150 mM NaCl, pH 7 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF) storage ring (Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.2 m and at a wavelength of λ = 0.102 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 25°C. 20 successive 0.600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

2-aminomuconic 6-semialdehyde dehydrogenase (AmnC mutant)
Mol. type   Protein
Organism   Pseudomonas sp.
Olig. state   Dimer
Mon. MW   50.4 kDa
 
UniProt   Q9KWS5 (1-476)
Sequence   FASTA
 
PDB ID   7BZV