Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome.

Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P, Int J Biochem Cell Biol 51:58-64 (2014) Europe PMC

SASDLH5 – Aggregation state of Ataxin-3 protein

Ataxin-3
MWI(0) 265 kDa
MWexpected 41 kDa
VPorod 425 nm3
log I(s) 1.29×102 1.29×101 1.29×100 1.29×10-1
Ataxin-3 small angle scattering data  s, nm-1
ln I(s)
Ataxin-3 Guinier plot ln 1.30×102 Rg: 6.9 nm 0 (6.9 nm)-2 s2
(sRg)2I(s)/I(0)
Ataxin-3 Kratky plot 1.104 0 √3 sRg
p(r)
Ataxin-3 pair distance distribution function Rg: 6.9 nm 0 Dmax: 25 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Ataxin-3 DAMMIN model
Synchrotron SAXS data from solutions of ataxin-3 protein in phosphate buffered saline (PBS), pH 7.4 were collected on the EMBL X33 beam line at DORIS III (DESY, Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.75 mg/ml was measured at 10°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Ataxin-3 (ATQ24)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   41.3 kDa
 
UniProt   P54252 (1-361)
Sequence   FASTA