| MWexperimental | 88 | kDa |
| MWexpected | 85 | kDa |
| VPorod | 160 | nm3 |
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log I(s)
3.28×10-2
3.28×10-3
3.28×10-4
3.28×10-5
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s, nm-1
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Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant
Metcalfe R Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M, Nature Communications 14(1) (2023) DOISASDLS3 – Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα
Interleukin-11 receptor subunit alphaInterleukin 11 Mutein
Interleukin-6 receptor subunit beta
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Fits and models
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Synchrotron SAXS
data from solutions of
Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα
in
20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH 8.5
were collected
on the
SAXS/WAXS beam line
at the Australian Synchrotron storage ring
(Melbourne, Australia)
using a Pilatus3 S 2M detector
at a sample-detector distance of 3.5 m and
at a wavelength of λ = 0.108 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 2 mg/ml was injected at a 45.00 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 20°C.
13 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample injection volume = UNKNOWN |
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