The conformational ensemble of Tau35

Stefano Da Vela.

SASDLS4 – Tau35, C-terminal fragment of human Tau protein

Microtubule-associated protein tau, Tau35 fragment
MWI(0) 19 kDa
MWexpected 27 kDa
VPorod 93 nm3
log I(s) 1.60×10-2 1.60×10-3 1.60×10-4 1.60×10-5
Microtubule-associated protein tau, Tau35 fragment small angle scattering data  s, nm-1
ln I(s)
Microtubule-associated protein tau, Tau35 fragment Guinier plot ln 1.60×10-2 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Microtubule-associated protein tau, Tau35 fragment Kratky plot 1.104 0 3 sRg
p(r)
Microtubule-associated protein tau, Tau35 fragment pair distance distribution function Rg: 4.9 nm 0 Dmax: 16.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Tau35, C-terminal fragment of human Tau protein Rg histogram Rg, nm

Synchrotron SAXS data from solutions of the C-terminal fragment of human Tau protein, Tau35, in phosphate buffered saline, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Microtubule-associated protein tau, Tau35 fragment (Tau35)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   26.8 kDa
 
UniProt   P10636 (504-758)
Sequence   FASTA