The conformational ensemble of Tau35

Stefano Da Vela.

SASDLU4 – Tau protein, 2N4R isoform

Microtubule-associated protein tau, 2N4R isoform
MWI(0) 36 kDa
MWexpected 46 kDa
VPorod 254 nm3
log I(s) 3.86×10-2 3.86×10-3 3.86×10-4 3.86×10-5
Microtubule-associated protein tau, 2N4R isoform small angle scattering data  s, nm-1
ln I(s)
Microtubule-associated protein tau, 2N4R isoform Guinier plot ln 3.86×10-2 Rg: 6.7 nm 0 (6.7 nm)-2 s2
(sRg)2I(s)/I(0)
Microtubule-associated protein tau, 2N4R isoform Kratky plot 1.104 0 3 sRg
p(r)
Microtubule-associated protein tau, 2N4R isoform pair distance distribution function Rg: 6.9 nm 0 Dmax: 23.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Tau protein, 2N4R isoform Rg histogram Rg, nm

Synchrotron SAXS data from solutions of the 2N4R isoform of Tau protein in phosphate buffered saline, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Microtubule-associated protein tau, 2N4R isoform (Tau441, 2N4R isoform)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   45.8 kDa
 
UniProt   P10636
Sequence   FASTA