Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation

Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J npj Biofilms and Microbiomes 8(1) (2022) DOI

SASDLW2 – Accessory colonization factor SslE at pH 4.4

Accessory colonization factor
MWexperimental 158 kDa
MWexpected 160 kDa
VPorod 248 nm3
log I(s) 2.06×10-1 2.06×10-2 2.06×10-3 2.06×10-4
Accessory colonization factor small angle scattering data  s, nm-1
ln I(s)
Accessory colonization factor Guinier plot ln 2.06×10-1 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Accessory colonization factor Kratky plot 1.104 0 √3 sRg
p(r)
Accessory colonization factor pair distance distribution function Rg: 4.0 nm 0 Dmax: 13.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Accessory colonization factor DAMMIN model
Synchrotron SAXS data from solutions of SslE in 20 mM citrate-phosphate buffer, 200 mM NaCl, pH 4.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403 column at 25°C. 620 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Accessory colonization factor (SslE)
Mol. type   Protein
Organism   Escherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / NCIMB 8666 / NRRL B-766 / W)
Olig. state   Monomer
Mon. MW   160.0 kDa
 
UniProt   E0IW31 (90-1520)
Sequence   FASTA