Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study

Schroer M, Hu P, Tomasovicova N, Batkova M, Zakutanska K, Wu P, Kopcansky P, Molecules 26(16):4864 (2021) DOI

SASDM24 – Fe3O4 nanoparticles (20 nm diameter)

Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter)
MWexperimental 1 kDa
MWexpected 1 kDa
log I(s) 1.75×107 1.75×106 1.75×105 1.75×104
Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) small angle scattering data  s, nm-1
ln I(s)
Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) Guinier plot ln 1.76×107 Rg: 11 nm 0 (11 nm)-2 s2
(sRg)2I(s)/I(0)
Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) Kratky plot 1.104 0 3 sRg
p(r)
Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) pair distance distribution function Rg: 8.7 nm 0 Dmax: 14 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Fe3O4 nanoparticles (20 nm diameter) in 50 mM borate buffer, 0.02% NaN3, pH 8.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Fe3O4 nanoparticles, coated with amphiphilic polymer coating composed of carboxylic acid atop an organic monomeric layer of oleic acid, dispersed in aqueous solution (purchased from Ocean Nano Inc., San Diego, CA, USA). In air measurement; temperature controlled capillary holder; Fe3O4 nanoparticle dispersion, with polydispersity: p(r) yields the volume weighted size distribution function for spherical particles. Average particle radius: 10.7 nm.

Fe3O4 nanoparticles; nominal diameter 20 nm (hydrodynamic diameter) (P20)
Mol. type   Other
Olig. state   Monomer
Mon. MW   1 kDa
Chemical formula