Structural basis for SHOC2 modulation of RAS signalling.

Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J, Nature (2022) Europe PMC

SASDMB5 – Leucine-rich repeat protein SHOC-2

Leucine-rich repeat protein SHOC-2

MW^experimental	58	kDa
MW^expected	65	kDa
V^Porod	85	nm³

log I(s) 1.22×10² 1.22×10¹ 1.22×10⁰ 1.22×10^-1

Leucine-rich repeat protein SHOC-2 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Leucine-rich repeat protein SHOC-2 Guinier plot

ln 1.22×10² R_g: 3.2 nm 0 (3.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

Leucine-rich repeat protein SHOC-2 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.4 nm 0 D_max: 11.2 nm

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Leucine-rich repeat protein SHOC-2 BILBOMD model

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of SHOC-2 in 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 28 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex LW-803 column at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Leucine-rich repeat protein SHOC-2
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Monomer
Mon. MW		65.1 kDa

UniProt		Q9UQ13 (2-582)
Sequence		FASTA