The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans.

Raczynska J, Olchowy J, Konariev PV, Svergun DI, Milewski S, Rypniewski W, J Mol Biol 372(3):672-88 (2007) Europe PMC

SASDMB7 – Glucosamine-6-phosphate Synthase from Candida albicans

N-acetylglucosamine kinase 1
MWexperimental 305 kDa
MWexpected 219 kDa
VPorod 421 nm3
log I(s) 3.92×104 3.92×103 3.92×102 3.92×101
N-acetylglucosamine kinase 1 small angle scattering data  s, nm-1
ln I(s)
N-acetylglucosamine kinase 1 Guinier plot ln 3.92×104 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
N-acetylglucosamine kinase 1 Kratky plot 1.104 0 √3 sRg
p(r)
N-acetylglucosamine kinase 1 pair distance distribution function Rg: 4.9 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
N-acetylglucosamine kinase 1 DAMMIN model
Synchrotron SAXS data from solutions of Glucosamine-6-phosphate Synthase from Candida albicans in 0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.30 mg/ml was measured at 25°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
N-acetylglucosamine kinase 1
Mol. type   Protein
Organism   Candida albicans (strain SC5314 / ATCC MYA-2876)
Olig. state   Tetramer
Mon. MW   54.8 kDa
 
UniProt   Q59RW5 (1-493)
Sequence   FASTA