| MWexperimental | 91 | kDa |
| MWexpected | 102 | kDa |
| VPorod | 132 | nm3 |
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log I(s)
2.01×102
2.01×101
2.01×100
2.01×10-1
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s, nm-1
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Structural basis for SHOC2 modulation of RAS signalling.
Liau NPD, Johnson MC, Izadi S, Gerosa L, Hammel M Bruning JM, Wendorff TJ, Phung W, Hymowitz SG, Sudhamsu J, Nature (2022) Europe PMCSASDMC5 – Leucine-rich repeat protein SHOC-2 bound to serine/threonine-protein phosphatase PP1-gamma catalytic subunit (PP1C)
Leucine-rich repeat protein SHOC-2Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
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Fits and models
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Synchrotron SAXS data from solutions of SHOC-2-PP1C in 25 mM Tris pH 7.5, 100 mM NaCl, 1 mM TCEP, 1 mM MnCl2, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 8 mg/ml was injected onto a column at 20°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN |
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