Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase

Daniel Murray.

SASDMK8 – Sulfite reductase flavoprotein octamer

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged)
MWexperimental 578 kDa
MWexpected 565 kDa
VPorod 830 nm3
log I(s) 1.21×100 1.21×10-1 1.21×10-2 1.21×10-3
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Guinier plot ln 1.22×100 Rg: 8.6 nm 0 (8.6 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) Kratky plot 1.104 0 3 sRg
p(r)
Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) pair distance distribution function Rg: 8.1 nm 0 Dmax: 26 nm

Data validation


There are no models related to this curve.

SANS data from solutions of sulfite reductase flavoprotein octamer in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). This sample is an octamer of His-tagged sulfite reductase flavoprotein subunits. Three instrument configurations were used: 9 m sample-to-detector distance with 1.5 nm wavelength band, 4 m sample-to-detector distance with 0.6 nm wavelength band, and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 3 hours / instrument configuration. Sample temperature: 8°C.

Sulfite reductase [NADPH] flavoprotein alpha-component (His-tagged) (His-tagged SiRFP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Octamer
Mon. MW   70.7 kDa
 
UniProt   P38038 (1-590)
Sequence   FASTA