| MWexperimental | 38 | kDa |
| MWexpected | 40 | kDa |
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log I(s)
3.27×101
3.27×100
3.27×10-1
3.27×10-2
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s, nm-1
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Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R, Journal of Virology 86(8):4444-4454 (2012) DOISASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)
Replicase polyprotein 1aReplicase polyprotein 1a
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Fits and models
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Synchrotron SAXS
data from solutions of
Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)
in
10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a Pilatus 1M-W detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.154 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 8.00 mg/ml was measured.
Eight successive
15 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN
Tags:
X33
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