Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori.

Tamrakar A, Singh R, Kumar A, Makde RD, Ashish, Kodgire P, Sci Rep 11(1):24471 (2021) Europe PMC

SASDML7 – HomB outer membrane protein

HomB outer membrane protein
MWexperimental 131 kDa
MWexpected 148 kDa
VPorod 500 nm3
log I(s) 2.94×10-1 2.94×10-2 2.94×10-3 2.94×10-4
HomB outer membrane protein small angle scattering data  s, nm-1
ln I(s)
HomB outer membrane protein Guinier plot ln 2.95×10-1 Rg: 8.2 nm 0 (8.2 nm)-2 s2
HomB outer membrane protein Kratky plot 1.104 0 3 sRg
Dmax: 27.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HomB outer membrane protein DAMMIN model

Synchrotron SAXS data from solutions of HomB protein in 20 mM Tris-HCl, 200 mM NaCl 5mM BME, pH 8 were collected on the BL-18 beam line at INDUS-2 (Indore, India) using a MAR 345 Image Plate detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.07749 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.5 mg/ml was measured at 37°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

CAUTION! Sample is likely aggregated. CAUTION! Guinier region ill-defined. CAUTION! Scattering intensity errors are over-estimated. CAUTION! Model likely does not represent a single particle.

HomB outer membrane protein (HomB)
Mol. type   Protein
Organism   Helicobacter pylori
Olig. state   Dimer
Mon. MW   74.2 kDa
Sequence   FASTA