Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase.

Murray DT Walia N, Weiss KL, Stanley CB, Randolph PS, Nagy G, Stroupe ME, Biophys J (2022) Europe PMC

SASDML8 – Sulfite reductase hemoprotein

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)
MWexperimental 65 kDa
MWexpected 64 kDa
VPorod 71 nm3
log I(s) 2.50×10-1 2.50×10-2 2.50×10-3 2.50×10-4
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Guinier plot ln 2.51×10-1 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Kratky plot 1.104 0 √3 sRg
p(r)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
SANS data from solutions of sulfite reductase hemoprotein in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) spallation neutron source (Oak Ridge, Tennessee, USA). Two instrument configurations were used: 4 m sample-to-detector distance with 0.6 nm wavelength band and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 5 mg/mL. Neutron exposure time: 1 hour / instrument configuration. Sample temperature: 8°C.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) (SiRHP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Monomer
Mon. MW   64.0 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA