| MWexperimental | 45 | kDa |
| MWexpected | 53 | kDa |
|
log I(s)
2.47×10-2
2.47×10-3
2.47×10-4
2.47×10-5
|
s, nm-1
|
Molecular and structural basis of olfactory sensory neuron axon coalescence by Kirrel receptors.
Wang J, Vaddadi N, Pak JS, Park Y, Quilez S, Roman CA, Dumontier E, Thornton JW, Cloutier JF, Özkan E, Cell Rep 37(5):109940 (2021) Europe PMCSASDMQ2 – Mouse Kirrel3 ectodomain - Q128A mutant
Kin of IRRE-like protein 3 (Q128A)|
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|
Fits and models
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Rg, nm
|
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Synchrotron SAXS
data from solutions of
Mouse Kirrel3 ectodomain - Q128A mutant
in
10 mM HEPES pH 7.2, 150 mM NaCl, pH 7.2
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 425.00 μl sample
at 2 mg/ml was injected at a 0.60 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 22°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. Number of frames = UNKNOWN |
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