Molecular and structural basis of olfactory sensory neuron axon coalescence by Kirrel receptors.

Wang J, Vaddadi N, Pak JS, Park Y, Quilez S, Roman CA, Dumontier E, Thornton JW, Cloutier JF, Özkan E, Cell Rep 37(5):109940 (2021) Europe PMC

SASDMQ2 – Mouse Kirrel3 ectodomain - Q128A mutant

Kin of IRRE-like protein 3 (Q128A)
MWexperimental 45 kDa
MWexpected 53 kDa
log I(s) 2.47×10-2 2.47×10-3 2.47×10-4 2.47×10-5
Kin of IRRE-like protein 3 (Q128A) small angle scattering data  s, nm-1
ln I(s)
Kin of IRRE-like protein 3 (Q128A) Guinier plot ln 2.47×10-2 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Kin of IRRE-like protein 3 (Q128A) Kratky plot 1.104 0 3 sRg
p(r)
Kin of IRRE-like protein 3 (Q128A) pair distance distribution function Rg: 5.9 nm 0 Dmax: 21.3 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Mouse Kirrel3 ectodomain - Q128A mutant Rg histogram Rg, nm
Kin of IRRE-like protein 3 (Q128A) EOM/RANCH model
Kin of IRRE-like protein 3 (Q128A) EOM/RANCH model
Kin of IRRE-like protein 3 (Q128A) EOM/RANCH model

Synchrotron SAXS data from solutions of Mouse Kirrel3 ectodomain - Q128A mutant in 10 mM HEPES pH 7.2, 150 mM NaCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 425.00 μl sample at 2 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Kin of IRRE-like protein 3 (Q128A) (Kirrel3 Q128A)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   53.0 kDa
 
UniProt   Q8BR86 (47-517)
Sequence   FASTA