X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB

Niemann H, Petoukhov M Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D, Journal of Molecular Biology 377(2):489-500 (2008) DOI

SASDMS4 – Listeria monocytogenes invasion protein internalin B

Internalin B
MWexperimental 33 kDa
MWexpected 36 kDa
VPorod 54 nm3
log I(s) 2.36×102 2.36×101 2.36×100 2.36×10-1
Internalin B small angle scattering data  s, nm-1
ln I(s)
Internalin B Guinier plot ln 2.37×102 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Internalin B Kratky plot 1.104 0 √3 sRg
p(r)
Internalin B pair distance distribution function Rg: 2.9 nm 0 Dmax: 9.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Internalin B PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Listeria monocytogenes invasion protein internalin B in 25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a 1D Gas detector detector at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 32.00 mg/ml was measured. 15 successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Internalin B (InlB)
Mol. type   Protein
Organism   Listeria monocytogenes serotype 1/2a (strain EGD / Mackaness)
Olig. state   Monomer
Mon. MW   35.8 kDa
 
UniProt   P0DQD3 (1-320)
Sequence   FASTA
 
PDB ID   2UZX