| MWexperimental | 131 | kDa |
| MWexpected | 136 | kDa |
| VPorod | 270 | nm3 |
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log I(s)
2.61×102
2.61×101
2.61×100
2.61×10-1
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s, nm-1
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X-ray and Neutron Small-Angle Scattering Analysis of the Complex Formed by the Met Receptor and the Listeria monocytogenes Invasion Protein InlB
Niemann H, Petoukhov M Härtlein M, Moulin M, Gherardi E, Timmins P, Heinz D, Svergun D, Journal of Molecular Biology 377(2):489-500 (2008) DOISASDMT4 – Complex of Met receptor with InlB
Hepatocyte growth factor receptorInternalin B
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Fits and models
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Synchrotron SAXS
data from solutions of
Complex of Met receptor with InlB
in
25mM Na-phosphate buffer, 150 mM NaCl, 1mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2 and 40 mg/ml were measured
.
15 successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN
Tags:
X33
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