Kinesin protein 3 B/B and kinesin associated protein 3 (KAP)

Kento Yonezawa.

SASDMU5 – Kinesin superfamily protein 3 B/B and kinesin associated protein 3 (KAP)

Kinesin-associated protein 3
Kinesin-like protein KIF3B
MWI(0) 226 kDa
MWexpected 145 kDa
VPorod 385 nm3
log I(s) 1.49×10-1 1.49×10-2 1.49×10-3 1.49×10-4
Kinesin-associated protein 3 Kinesin-like protein KIF3B small angle scattering data  s, nm-1
ln I(s)
Kinesin-associated protein 3 Kinesin-like protein KIF3B Guinier plot ln 1.49×10-1 Rg: 5.7 nm 0 (5.7 nm)-2 s2
(sRg)2I(s)/I(0)
Kinesin-associated protein 3 Kinesin-like protein KIF3B Kratky plot 1.104 0 √3 sRg
p(r)
Kinesin-associated protein 3 Kinesin-like protein KIF3B pair distance distribution function Rg: 6.2 nm 0 Dmax: 28 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Kinesin-associated protein 3 Kinesin-like protein KIF3B DAMMIF model
Synchrotron SAXS data from solutions of Kinesin superfamily protein 3 B/B and Kinesin associated protein 3 in 20 mM TrisHCl, 200 mM NaCl, 1mM DTT pH 8 were collected on the beam line BL-10C at the Phton Factory (PF; Tsukuba, Ibaraki, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.65 m and at a wavelength of λ = 0.1213 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 330 successive 20 second frames were collected at 20°C using size-exclusion chromatography SAXS. Sample was loaded to Superdex 200 increase 10/300 (GE healthcare). Flow rate was set at 0.05 mL/min. The data were normalized to the intensity of the incident beam and circularly averaged; the scattering of the solvent-blank was subtracted. The extrapolated scattering profile at a concentration of zero was calculated with the data in the descent side of the peak (frames 176–199) by Serial Analyzer. In order to estimate the molecular mass from I(0), PSV (0.738 cm3/g) and the scattering mass contrast (2.707×10^10 cm-2) of this protein were calculated by using MULCh (http://smb-research.smb.usyd.edu.au/NCVWeb/input.jsp).

Kinesin-associated protein 3 (KAP 3)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   81.1 kDa
 
UniProt   P70188 (1-693)
Sequence   FASTA
 
Kinesin-like protein KIF3B (KIF 3 B)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   31.9 kDa
 
UniProt   Q61771 (475-747)
Sequence   FASTA