Neutron scattering maps the higher-order assembly of NADPH-dependent assimilatory sulfite reductase

Daniel Murray.

SASDMU8 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 86% D2O

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein)
MWexperimental 136 kDa
MWexpected 125 kDa
log I(s) 7.26×10-2 7.26×10-3 7.26×10-4 7.26×10-5
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) small angle scattering data  s, nm-1
ln I(s)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) Guinier plot ln 7.27×10-2 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) Kratky plot 1.104 0 3 sRg
p(r)
Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) pair distance distribution function Rg: 3.4 nm 0 Dmax: 12.4 nm

Data validation


There are no models related to this curve.

SANS data from solutions of sulfite reductase flavoprotein-60/deuterated-hemoprotein heterodimer in 86% D2O, 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 Two instrument configurations were used: 4 m sample-to-detector distance with 0.6 nm wavelength band and 1.3 m sample-to-detector distance with 0.4 nm wavelength band. Sample concentration: 3 mg/mL. Neutron exposure time: 2 hours / instrument configuration. Sample temperature: 8°C.

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) (SiRHP)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Monomer
Mon. MW   64.0 kDa
 
UniProt   P17846 (1-570)
Sequence   FASTA
 
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) (SiRFP-60)
Mol. type   Protein
Organism   Escherichia coli (strain K12)
Olig. state   Monomer
Mon. MW   60.7 kDa
 
UniProt   P38038 (53-599)
Sequence   FASTA