Dependence of the Nanoscale Composite Morphology of Fe3O4 Nanoparticle-Infused Lysozyme Amyloid Fibrils on Timing of Infusion: A Combined SAXS and AFM Study

Schroer M, Hu P, Tomasovicova N, Batkova M, Zakutanska K, Wu P, Kopcansky P, Molecules 26(16):4864 (2021) DOI

SASDMY3 – Lysozyme amyloid fibril (LAF)

lysozyme amyloid fibril
MWexperimental 1 kDa
MWexpected 1 kDa
log I(s) 3.68×107 3.68×106 3.68×105 3.68×104
lysozyme amyloid fibril small angle scattering data  s, nm-1
ln I(s)
lysozyme amyloid fibril Guinier plot ln 3.68×107 Rg: 38.4 nm 0 (38.4 nm)-2 s2
lysozyme amyloid fibril Kratky plot 1.104 0 3 sRg
lysozyme amyloid fibril pair distance distribution function Rg: 24.3 nm 0 Dmax: 80 nm

Data validation

Fits and models

log I(s)
 s, nm-1
lysozyme amyloid fibril DAMMIF model

Synchrotron SAXS data from solutions of Lysozyme amyloid fibril (LAF) in 0.2 M glycine-HCl, 80 mM NaCl, pH 2.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

LAF solution commenced with the dissolving of the hen egg white lysozyme (HEWL) powder in the buffer solution that consisted of 0.2 M glycine-HCl, with a pH of 2.2 and 80 mM NaCl, settling down at the concentration of 5 mg/mL. The buffer solution was conditioned at the acidic pH level of 2.2 for breaking down of the HEWL powder within a short period of time relative to the naturally occurring process.. In-air SAXS measurements. Set-up: Temperature controlled capillary holder. LAFs are too large to be properly resolve the Guiner range with a camera length of 3.0 m and 10 keV.

lysozyme amyloid fibril (LAF)
Mol. type   Protein
Organism   Gallus gallus
Olig. state   Unknown
Mon. MW   1 kDa
UniProt   P00698
Sequence   FASTA