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Synchrotron SAXS
data from solutions of
Lysozyme amyloid fibril (LAF)
in
0.2 M glycine-HCl, 80 mM NaCl, pH 2.2
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.123 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
at 20°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
LAF solution commenced with the dissolving of the hen egg white lysozyme (HEWL) powder in the buffer solution that consisted of 0.2 M glycine-HCl, with a pH of 2.2 and 80 mM NaCl, settling down at the concentration of 5 mg/mL. The buffer solution was conditioned at the acidic pH level of 2.2 for breaking down of the HEWL powder within a short period of time relative to the naturally occurring process..
In-air SAXS measurements. Set-up: Temperature controlled capillary holder. LAFs are too large to be properly resolve the Guiner range with a camera length of 3.0 m and 10 keV.
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s, nm-1
