Synchrotron SAXS data from solutions of Heparin oligosaccharide DP10 in 10 mM HEPES, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 4 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Three serial dilutions of DP10 in HBS were collected and extrapolated to have zero concentration. All the calculations were done in ATSAS. These oligosaccharides have been prepared by high resolution gel filtration of partial heparin lyase digestion of high quality heparin.
General formula*
∆HexA,2S - GlcNS,6S– (IdoUA,2S – GlcNS,6S)n
n = number of disaccharide units.
*Although the main disaccharide unit in these products is IdoUA,2S – GlcNS,6S, (approx 75%) saccharides in each size class show some variation in degree and pattern of sulphation.
Uronic acid (HexA) at the non-reducing end of the oligosaccharides has a C4-C5 double bond as a result of the endolytic action of bacterial heparin lyase.
s, nm-1
