NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin.

Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A, Nucleic Acids Res (2022) Europe PMC

SASDN36 – Ox40 RNA (untranslated region, UTR) full-length

Ox40 3'UTR full-length
MWexperimental 48 kDa
MWexpected 50 kDa
VPorod 115 nm3
log I(s) 1.10×10-1 1.10×10-2 1.10×10-3 1.10×10-4
Ox40 3'UTR full-length small angle scattering data  s, nm-1
ln I(s)
Ox40 3'UTR full-length Guinier plot ln 1.11×10-1 Rg: 3.7 nm 0 (3.7 nm)-2 s2
Ox40 3'UTR full-length Kratky plot 1.104 0 3 sRg
Dmax: 12 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Ox40 RNA (untranslated region, UTR) full-length in 50 mM KCl, 25 mM sodium phosphate, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.43 mg/ml was measured at 20°C. 59 successive 0.145 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ox40 3'UTR full-length
Mol. type   RNA
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   50.3 kDa
Sequence   FASTA