| MWexperimental | 318 | kDa |
| MWexpected | 270 | kDa |
| VPorod | 430 | nm3 |
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log I(s)
1.67×10-1
1.67×10-2
1.67×10-3
1.67×10-4
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s, nm-1
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The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength
Guerra J Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A, International Journal of Molecular Sciences 23(9):4871 (2022) DOISASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0
DNA protection during starvation, DPS (Ferritin superfamily)|
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Fits and models
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Synchrotron SAXS data from solutions of DgrDpsWT in 50 mM MOPS-NaOH, 480 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 15°C. 40 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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