The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength

Guerra J Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A, International Journal of Molecular Sciences 23(9):4871 (2022) DOI

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily)
MWexperimental 208 kDa
MWexpected 218 kDa
VPorod 291 nm3
log I(s) 4.67×104 4.67×103 4.67×102 4.67×101
DNA protection during starvation, DPS-∆N (Ferritin superfamily) small angle scattering data  s, nm-1
ln I(s)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) Guinier plot ln 4.67×104 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) Kratky plot 1.104 0 √3 sRg
p(r)
DNA protection during starvation, DPS-∆N (Ferritin superfamily) pair distance distribution function Rg: 3.7 nm 0 Dmax: 8.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA protection during starvation, DPS-∆N (Ferritin superfamily) DAMMIN model
Synchrotron SAXS data from solutions of DgrDps∆N in 50 mM MOPS-NaOH, 230 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 15°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA protection during starvation, DPS-∆N (Ferritin superfamily) (DgrDps∆N)
Mol. type   Protein
Organism   Deinococcus grandis
Olig. state   Dodecamer
Mon. MW   18.2 kDa
 
UniProt   A0A100HLL5 (56-214)
Sequence   FASTA