Synchrotron SAXS data from solutions of Heparin oligosaccharide DP18 in 50 mM Tris/HCl, pH 7.4 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.25 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Serial dilutions of DP18 were performed from 5 mg/ml to 0.625 mg/ml. In absence of salt there high degree of sulfation of the sample produced interparticle interference in the absence of salts. These oligosaccharides have been prepared by high resolution gel filtration of partial heparin lyase (Heparinase I) digestion of high quality heparin.
General formula*
∆HexA,2S - GlcNS,6S– (IdoUA,2S – GlcNS,6S)n
n = number of disaccharide units.
*Although the main disaccharide unit in these products is IdoUA,2S – GlcNS,6S, (approx 75%) saccharides in each size class show some variation in degree and pattern of sulphation.
Uronic acid (HexA) at the non-reducing end of the oligosaccharides has a C4-C5 double bond as a result of the endolytic action of bacterial heparin lyase.
s, nm-1
