Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate

Adriana Erica Miele.

SASDN94 – Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan
MWexperimental 6 kDa
MWexpected 5 kDa
log I(s) 2.71×10-2 2.71×10-3 2.71×10-4 2.71×10-5
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan small angle scattering data  s, nm-1
ln I(s)
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Guinier plot ln 2.72×10-2 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan Kratky plot 1.104 0 √3 sRg
p(r)
Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan pair distance distribution function Rg: 2 nm 0 Dmax: 7.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Heparin oligosaccharide DP18 in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

These oligosaccharides have been prepared by high resolution gel filtration of partial heparin lyase (Heparinase I) digestion of high quality heparin. General formula* ∆HexA,2S - GlcNS,6S– (IdoUA,2S – GlcNS,6S)n n = number of disaccharide units. *Although the main disaccharide unit in these products is IdoUA,2S – GlcNS,6S, (approx 75%) saccharides in each size class show some variation in degree and pattern of sulphation. Uronic acid (HexA) at the non-reducing end of the oligosaccharides has a C4-C5 double bond as a result of the endolytic action of bacterial heparin lyase.

Heparine oligosaccharide of 18 sugar units (9 disaccharides), digested by Heparinase I from purified HP glycosaminoglycan (DP18)
Mol. type   Other
Organism   Sus scrofa domesticus
Olig. state   Monomer
Mon. MW   5.2 kDa
Chemical formula