Thyroglobulin - human and bovine SAXS/WAXS data

James Byrnes.

SASDNA7 – Bovine thyroglobulin in phosphate buffered saline/NaCl

MWexperimental 527 kDa
MWexpected 606 kDa
VPorod 1950 nm3
log I(s) 6.13×101 6.13×100 6.13×10-1 6.13×10-2
Thyroglobulin small angle scattering data  s, nm-1
ln I(s)
Thyroglobulin Guinier plot ln 6.14×101 Rg: 8.1 nm 0 (8.1 nm)-2 s2
Thyroglobulin Kratky plot 1.104 0 3 sRg
Thyroglobulin pair distance distribution function Rg: 8.4 nm 0 Dmax: 29.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Thyroglobulin DAMMIF model
Thyroglobulin DAMFILT model

Synchrotron SAXS data from solutions of thyroglobulin in phosphate buffered saline, 150mM NaCl, pH 8 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 3.3 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column. 360 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray wavelength = UNKNOWN. Experimental temperature = UNKNOWN. Sample to detector distance(s): UNKNOWN. The bead models in this entry represent an individual model with the associated fit to the data (top) and; the volume/bead occupancy-corrected averaged representation of the protein in solution generated from a spatial alignment of an individual model cohort (bottom).

Mol. type   Protein
Organism   Bos taurus
Olig. state   Dimer
Mon. MW   303.2 kDa
UniProt   P01267 (1-2769)
Sequence   FASTA