Synchrotron SAXS data from solutions of the filamentous assembly of NrdR in 100 mM NaCl, 20 mM Tris, 1 mM DTT, 3% v/v glycerol, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.40 mg/ml was measured at 20°C. 62 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Appears as a large filamentous assembly, therefore the molecular weight is indeterminate, ranging above 500 kDa. The recombinant protein contains a N-terminal hexa-histidine amino acid protein affinity chromatography tag (HHHHHHLE). The p(r) profile estimation of Dmax should be interpreted only as an approximation.