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Synchrotron SAXS data from solutions of a secreted form that lacks the transmembrane domain of mouse LARGE xylosyl- and glucuronyltransferase 2 (LARGE2dTM) in buffer (20 mM HEPES pH 7.4, 150 mM NaCl) were collected on the BioCAT 18-ID-D beamline at the Advanced Photon Source (APS) (Chicago, IL, USA) using a Eiger2 XE 9M detector at a sample-detector distance of 3.67 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC)-MALS-SAXS was employed. The SEC parameters were as follows: A 300 μl sample at 4 mg/ml was injected at a 0.6 ml/min flow rate onto a Superdex 200 increase 10/300 GL column (GE healthcare) at 23°C. 2500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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s, nm-1
