| MWexperimental | 183 | kDa |
| MWexpected | 183 | kDa |
| VPorod | 500 | nm3 |
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log I(s)
1.78×10-2
1.78×10-3
1.78×10-4
1.78×10-5
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s, nm-1
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Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation
Hsu J, Chen J, Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M Frontiers in Molecular Biosciences 9 (2022) DOISASDNJ6 – full-length MERS CoV N-protein complexed with 5-(2-methoxyethoxy)-1H-indole (P4-4 compound)
Nucleoprotein5-(2-methoxyethoxy)-1H-indole
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Fits and models
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Synchrotron SAXS
data from solutions of
full-length MERS CoV N-protein complexed with 5-(2-methoxyethoxy)-1H-indole (P4-4 compound)
in
50 mM Tris-HCl, 150 mM NaCl, pH 8.5
were collected
on the
23A beam line
at the Taiwan Photon Source, NSRRC storage ring
(Hsinchu, Taiwan)
using a Pilatus 1M-F detector
at a sample-detector distance of 2.5 m and
at a wavelength of λ = 0.08 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample
at 2 mg/ml was injected at a 0.02 ml/min flow rate
onto a Agilent Bio SEC-3, 300 Å column
at 14.9°C.
36 successive
30 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Quoted experimental molecular weight (183 kDa) cannot be verified from the data. The Dmax is likely underestimated. |
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