Relative domain orientation of the L289K HIV ‐1 reverse transcriptase monomer

Xi Z, Ilina T, Guerrero M, Fan L, Sluis‐Cremer N, Wang Y, Ishima R Protein Science 31(5) (2022) DOI

SASDNL3 – HIV-1 reverse transcriptase L289K mutation (extrapolated to zero concentration)

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556)
MWI(0) 62 kDa
MWexpected 65 kDa
VPorod 90 nm3
log I(s) 1.31×10-1 1.31×10-2 1.31×10-3 1.31×10-4
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) small angle scattering data  s, nm-1
ln I(s)
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Guinier plot ln 1.32×10-1 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) Kratky plot 1.104 0 √3 sRg
p(r)
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) pair distance distribution function Rg: 3.2 nm 0 Dmax: 11.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) BUNCH model
Synchrotron SAXS data from solutions of HIV-1 reverse transcriptase (C38V/C280S, L289K) in 50 mM Bis-Tris pH 6.8, 100 mM NaCl, 1% glycerol, 0.02% w/v NaN3, pH 6.8 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 1.9 m and at a wavelength of λ = 0.08856 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 30 successive 1.500 second frames were collected at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The resulting data is that calculated from the extrapolation of a concentration series SAXS data set to zero concentration.

Concentration = UNKNOWN

Gag-Pol polyprotein (HIV-1 reverse transcriptase C38V/C280S, L289K truncated to 1 - 556) (p66_L289K)
Mol. type   Protein
Organism   Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)
Olig. state   Monomer
Mon. MW   64.7 kDa
 
UniProt   P04585 (588-1143)
Sequence   FASTA