Synchrotron SAXS data from solutions of Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, pH 7.4 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.25 mg/ml was measured at 20°C. 20 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Heparan sulphate isolated from porcine mucosa. This material contains both high and low sulphated species of HS and gives a bimodal molecular weight profile with fractions of approx. 40 kDa and 9 kDa; the smaller of the two fractions is the more sulphated component.
s, nm-1
