| MWexperimental | 58 | kDa |
| MWexpected | 44 | kDa |
| VPorod | 102 | nm3 |
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log I(s)
2.10×101
2.10×100
2.10×10-1
2.10×10-2
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s, nm-1
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Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation.
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C, Matrix Biol (2022) Europe PMCSASDNS3 – Latent-transforming growth factor beta-binding protein 1 C-terminus
Latent-transforming growth factor beta-binding protein 1|
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Fits and models
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Synchrotron SAXS data from solutions of latent-transforming growth factor beta-binding protein 1 (C-terminus) in Tris buffered saline, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 3 mg/ml was injected at a 0.05 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column. 2000 successive 1 second frames were collected where SAXS data frames 1391 - 1469 were used for processing. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN |
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