| MWexperimental | 318 | kDa |
| MWexpected | 122 | kDa |
| VPorod | 759 | nm3 |
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log I(s)
3.50×101
3.50×100
3.50×10-1
3.50×10-2
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s, nm-1
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Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation.
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C, Matrix Biol (2022) Europe PMCSASDNT3 – Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 C-terminus by transglutaminase 2
Latent-transforming growth factor beta-binding protein 1Fibrillin-1 PF3
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Fits and models
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Synchrotron SAXS data from solutions of Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 (C-terminus) in 10 mM Hepes, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 3 mg/ml was injected onto a column. 2000 successive 1 second frames were collected where SAXS data frames 1108 - 1215 were used for processing. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN |
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