The N-terminus of obscurin is flexible in solution.

Mauriello GE, Moncure GE, Nowzari RA, Miller CJ, Wright NT, Proteins (2022) Europe PMC

SASDNU7 – Obscurin Ig domains 12/13 at neutral pH

MWexperimental 21 kDa
MWexpected 21 kDa
VPorod 28 nm3
log I(s) 8.53×100 8.53×10-1 8.53×10-2 8.53×10-3
Obscurin small angle scattering data  s, nm-1
ln I(s)
Obscurin Guinier plot ln 8.53×100 Rg: 2.8 nm 0 (2.8 nm)-2 s2
Obscurin Kratky plot 1.104 0 3 sRg
Dmax: 10.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Obscurin Ig domains 12/13 at neutral pH in 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time: UNKNOWN.

Obscurin (Obscurin Ig 12-13)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   21.0 kDa
UniProt   Q5VST9 (1071-1254)
Sequence   FASTA