Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface

Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K, Journal of Molecular Biology 377(1):206-219 (2008) DOI

SASDNX2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand

Fab fragment in complex with small molecule hapten, crystal form-1
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL
MWexperimental 92 kDa
MWexpected 45 kDa
VPorod 139 nm3
log I(s) 1.70×102 1.70×101 1.70×100 1.70×10-1
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL small angle scattering data  s, nm-1
ln I(s)
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL Guinier plot ln 1.71×102 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL Kratky plot 1.104 0 √3 sRg
p(r)
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL pair distance distribution function Rg: 4.7 nm 0 Dmax: 16 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL DAMMIN model
log I(s)
 s, nm-1
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Fab fragment in complex with small molecule hapten, crystal form-1 (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand in 20 mM Tris–HCl, 50 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.70 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Tags: X33
Fab fragment in complex with small molecule hapten, crystal form-1 (Fab)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   45.3 kDa
Sequence   FASTA
 
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL (TSC)
Mol. type   Other
Olig. state   Monomer
Mon. MW   0.2 kDa
Chemical formula
 
PDB ID   2JB6