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Synchrotron SAXS data from solutions of the complex formed between the nuclear receptor subfamily proteins PXR and CAR in 25 mM HEPES, 150 mM NaCl, 5% glycerol, 5 mM DTT, pH 7.9 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source (NSLS-II; Brookhaven, NY, USA) using a Pilatus3 S 1M detector at a wavelength of λ = 0.081 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 65.00 μl sample was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Data were recorded at two detector positions, SAXS and WAXS, and subsequently merged.
The PXR protein construct contains a tethered segment at the C-terminus. The CAR protein construct contains a tethered segment at the C-terminus and relates to isoform 5 (UniProt Q14994-5).
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s, nm-1
