| MWexperimental | 242 | kDa |
| MWexpected | 270 | kDa |
| VPorod | 475 | nm3 |
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log I(s)
2.03×104
2.03×103
2.03×102
2.03×101
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s, nm-1
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The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength
Guerra J Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A, International Journal of Molecular Sciences 23(9):4871 (2022) DOISASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0
DNA protection during starvation, DPS (Ferritin superfamily)|
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Fits and models
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Synchrotron SAXS data from solutions of DgrDpsWT in 50 mM MOPS-NaOH, 80 mM NaCl, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 15°C. 3000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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