| MWI(0) | 42850 | kDa |
| MWexpected | 46 | kDa |
| VPorod | 58 | nm3 |
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log I(s)
6.07×106
6.07×105
6.07×104
6.07×103
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s, nm-1
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Glycoside hydrolase subfamily GH5_57 features a highly redesigned catalytic interface to process complex hetero-β-mannans.
Martins MP, Morais MAB, Persinoti GF, Galinari RH, Yu L, Yoshimi Y, Passos Nunes FB, Lima TB, Barbieri SF, Silveira JLM, Lombard V, Terrapon N, Dupree P, Henrissat B, Murakami MT, Acta Crystallogr D Struct Biol 78(Pt 11):1358-1372 (2022) Europe PMCSASDP46 – Glycoside Hydrolase Family 5 endo-mannanase retrieved from Capybara gut metagenome
GH5 endo-mannanase from Capybara gut metagenome|
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Fits and models
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Synchrotron SAXS data from solutions of glycoside hydrolase family 5 endo-mannanase retrieved from Capybara gut metagenome in 20 mM HEPES, 150 mM NaCl, pH 7.4 were collected on the SAXS1 Beamline beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a Pilatus 300K detector at a sample-detector distance of 0.1 m and at a wavelength of λ = 0.145 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured. 10 successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN |
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