DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates.

Zhao L Huang N, Mencius J, Li Y, Xu Y, Zheng Y, He W, Li N, Zheng J, Zhuang M, Quan S, Chen Y, iScience 25(9):104948 (2022) Europe PMC

SASDPU3 – Histone-lysine N-methyltransferase multi protein complex bound to protein dpy-30 homologue: MLL1-WDR5-RBBP5-ASH2L-DPY30

Histone-lysine N-methyltransferase 2A
WD repeat-containing protein 5
Retinoblastoma-binding protein 5
Set1/Ash2 histone methyltransferase complex subunit ASH2
Protein dpy-30 homolog
MWexperimental 208 kDa
MWexpected 203 kDa
VPorod 411 nm3
log I(s) 1.95×102 1.95×101 1.95×100 1.95×10-1
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog small angle scattering data  s, nm-1
ln I(s)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Guinier plot ln 1.96×102 Rg: 5.4 nm 0 (5.4 nm)-2 s2
(sRg)2I(s)/I(0)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Kratky plot 1.104 0 3 sRg
p(r)
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog pair distance distribution function Rg: 5.5 nm 0 Dmax: 17.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of MLL1-WDR5-RBBP5-ASH2L-DPY30 in 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.09184 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histone-lysine N-methyltransferase 2A (MLL1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   24.9 kDa
 
UniProt   Q03164 (3754-3969)
Sequence   FASTA
 
WD repeat-containing protein 5 (WDR5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   36.6 kDa
 
UniProt   P61964 (1-334)
Sequence   FASTA
 
Retinoblastoma-binding protein 5 (RBBP5)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   59.2 kDa
 
UniProt   Q15291 (1-538)
Sequence   FASTA
 
Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   60.2 kDa
 
UniProt   Q9UBL3-3 (95-534)
Sequence   FASTA
 
Protein dpy-30 homolog (DPY30)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   11.3 kDa
 
UniProt   Q9C005 (1-99)
Sequence   FASTA