MHV Coronavirus Packaging Signal RNA and N protein

Suzette Pabit.

SASDPW5 – Murine hepatitis virus (MHV) coronavirus putative packaging signal RNA (SAXS/WAXS)

MHV coronavirus putative packaging signal RNA
MWexperimental 30 kDa
MWexpected 31 kDa
VPorod 58 nm3
log I(s) 6.56×101 6.56×100 6.56×10-1 6.56×10-2
MHV coronavirus putative packaging signal RNA small angle scattering data  s, nm-1
ln I(s)
MHV coronavirus putative packaging signal RNA Guinier plot ln 6.57×101 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
MHV coronavirus putative packaging signal RNA Kratky plot 1.104 0 √3 sRg
p(r)
MHV coronavirus putative packaging signal RNA pair distance distribution function Rg: 4.0 nm 0 Dmax: 13.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of MHV coronavirus putative packaging signal RNA in 50 mM NaCl, 50 μM EDTA, 10 mM MOPS, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus3 S 1M detector at a wavelength of λ = 0.0918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 3 mg/ml were measured at 10°C. Five successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Data collected from two detector positions were merged to obtain the SAXS/WAXS profile.

MHV coronavirus putative packaging signal RNA (MHV-PS-RNA)
Mol. type   RNA
Organism   Murine coronavirus
Olig. state   Monomer
Mon. MW   31.5 kDa
Sequence   FASTA