MHV Coronavirus Packaging Signal RNA and N protein

Suzette Pabit.

SASDPX5 – Murine hepatitis virus (MHV) coronavirus nucleocapsid protein N

Nucleoprotein
MWexperimental 50 kDa
MWexpected 50 kDa
VPorod 91 nm3
log I(s) 1.56×10-2 1.56×10-3 1.56×10-4 1.56×10-5
Nucleoprotein small angle scattering data  s, nm-1
ln I(s)
Nucleoprotein Guinier plot ln 1.56×10-2 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoprotein Kratky plot 1.104 0 3 sRg
p(r)
Nucleoprotein pair distance distribution function Rg: 4.2 nm 0 Dmax: 11.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of MHV coronavirus nucleocapsid protein N in 70 mM KCl, 50 μM EDTA, 25 mM HEPES, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA) using a Eiger 4M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.1265 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 0.5 mg/ml were measured at 22°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Nucleoprotein
Mol. type   Protein
Organism   Murine coronavirus (strain A59)
Olig. state   Monomer
Mon. MW   49.7 kDa
 
UniProt   P03416 (1-454)
Sequence   FASTA