DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling.

Mao YQ, Seraphim TV, Wan Y, Wu R, Coyaud E, Bin Munim M, Mollica A, Laurent E, Babu M, Mennella V, Raught B, Houry WA, Cell Rep 43(2):113713 (2024) Europe PMC

SASDQ98 – Full-length human DPCD

Protein DPCD
MWexperimental 20 kDa
MWexpected 23 kDa
VPorod 28 nm3
log I(s) 1.01×101 1.01×100 1.01×10-1 1.01×10-2
Protein DPCD small angle scattering data  s, nm-1
ln I(s)
Protein DPCD Guinier plot ln 1.02×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Protein DPCD Kratky plot 1.104 0 √3 sRg
p(r)
Protein DPCD pair distance distribution function Rg: 2.1 nm 0 Dmax: 7.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Full-length human DPCD Rg histogram Rg, nm
Protein DPCD EOM/RANCH model
Protein DPCD EOM/RANCH model
Protein DPCD EOM/RANCH model
Protein DPCD EOM/RANCH model
Protein DPCD EOM/RANCH model
log I(s)
 s, nm-1
Protein DPCD GASBOR model
log I(s)
 s, nm-1
Protein DPCD ALPHAFOLD model
Synchrotron SAXS data from solutions of full-length human DPCD in 25 mM Tris-HCl, 200 mM NaCl, pH 7.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II; Upton, NY, USA) using a Pilatus / 1M SAXS detector detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.0819 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.2 and 3.6 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The data from the concentration series were extrapolated to infinite dilution.

DPCD data were collected from samples prepared at 0.23 mg/mL, 0.45 mg/mL, 0.9 mg/mL, 1.8 mg/mL, and 3.6 mg/mL in 25 mM Tris-HCl (pH 7.5), 200 mM NaCl. A single curve extrapolated to infinite dilution was obtained using Almerge in the ATSAS 2.7.2 package. Recombinant human DPCD was heterologously expressed in Escherichia coli.

Protein DPCD (DPCD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   23.2 kDa
 
UniProt   Q9BVM2 (1-203)
Sequence   FASTA