| MWexperimental | 240 | kDa |
| MWexpected | 239 | kDa |
| VPorod | 326 | nm3 |
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log I(s)
2.96×10-1
2.96×10-2
2.96×10-3
2.96×10-4
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s, nm-1
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Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase.
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M Severinov K, Isaev A, Cell Rep 42(8):112972 (2023) Europe PMCSASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3
S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A)
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Fits and models
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Synchrotron SAXS data from solutions of the complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3 in 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.09998 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.6 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403 column at 25°C. 12 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Rg and MW calculated using SAXSMoW: http://saxs.ifsc.usp.br |
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