| MWexperimental | 240 | kDa |
| MWexpected | 239 | kDa |
| VPorod | 326 | nm3 |
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log I(s)
2.96×10-1
2.96×10-2
2.96×10-3
2.96×10-4
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s, nm-1
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Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase.
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A, Cell Rep 42(8):112972 (2023) Europe PMCSASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3
S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A)
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Fits and models
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Synchrotron SAXS data from solutions of the complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3 in 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.09998 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 9.6 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403 column at 25°C. 12 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Rg and MW calculated using SAXSMoW: http://saxs.ifsc.usp.br |
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