Synchrotron SAXS data from solutions of GRP94 protein bound to AMP-PNP in 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility, (SSRF, Shanghai, China) using a Pilatus 1 M detector detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 10 mg/ml was injected at a 0.50 ml/min flow rate onto a Wyatt WTC-030S5 5μm 300+, 7.8x300 mm column at 25°C. 720 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
