Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity.

Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S, Signal Transduct Target Ther 8(1):445 (2023) Europe PMC

SASDQV9 – Nanobody1 in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17
Nanobody1
MWexperimental 40 kDa
MWexpected 20 kDa
VPorod 52 nm3
log I(s) 1.02×100 1.02×10-1 1.02×10-2 1.02×10-3
Tumor necrosis factor receptor superfamily member 17 Nanobody1 small angle scattering data  s, nm-1
ln I(s)
Tumor necrosis factor receptor superfamily member 17 Nanobody1 Guinier plot ln 1.02×100 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Tumor necrosis factor receptor superfamily member 17 Nanobody1 Kratky plot 1.104 0 √3 sRg
p(r)
Tumor necrosis factor receptor superfamily member 17 Nanobody1 pair distance distribution function Rg: 3.0 nm 0 Dmax: 9.8 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of nanobody1 in complex with the extracellular domain of human BCMA in 20 mM Tris, 100 mM NaCl, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 25°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tumor necrosis factor receptor superfamily member 17 (BCMA-ECD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   5.9 kDa
 
UniProt   Q02223 (1-54)
Sequence   FASTA
 
Nanobody1 (Nb1)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   13.9 kDa
Sequence   FASTA