Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A

Bochel A, Mortensen S, Seifert L, Hengel F, Jeffries C, Chojnowski G, Kretz O, Huber T, Tomas N, Wilmanns M, (2023) DOI

SASDR27 – Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A

Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A)
MWexperimental 14 kDa
MWexpected 16 kDa
VPorod 19 nm3
log I(s) 1.61×10-2 1.61×10-3 1.61×10-4 1.61×10-5
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) small angle scattering data  s, nm-1
ln I(s)
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) Guinier plot ln 1.61×10-2 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) Kratky plot 1.104 0 √3 sRg
p(r)
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) pair distance distribution function Rg: 2.8 nm 0 Dmax: 11.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Triple mutant (D116A, H118A, Y176A) of the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A Rg histogram Rg, nm
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) OTHER [STATIC IMAGE] model
log I(s)
 s, nm-1
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) SASREF model
log I(s)
 s, nm-1
Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) GASBOR model
Synchrotron SAXS data from solutions of a triple alanine mutant in the D1-D2 N-terminal domains of thrombospondin type-1 domain-containing protein 7A in 25 mM Tris, 150 mM NaCl, 5 mM NaNO3, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 4.1 mg/ml was injected at a 0.35 ml/min flow rate onto a GE Superdex 75 Increase 5/150 column at 20°C. 91 successive 0.240 second frames were collected through the SEC-elution peak and processed using CHROMIXS. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Additional SEC-SAXS data (unsubtracted data frames), CHROMIXS Rg correlations, and SASREF/GASBOR models in addition to EOM outputs are made available in the full entry zip archive

Thrombospondin type-1 domain-containing protein 7A (D116A, H118A, Y176A) (THSD7A-mutant)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   16.5 kDa
 
UniProt   Q9UPZ6 (48-192)
Sequence   FASTA
 
ALPHAFOLD ID   Q9UPZ6