Decoding optimal ligand design for multicomponent condensates

Sarasi Galagedera.

SASDR97 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin

MWI(0) 51 kDa
MWexpected 51 kDa
VPorod 73 nm3
log I(s) 2.66×10-2 2.66×10-3 2.66×10-4 2.66×10-5
HOTag-PA-Ubiquitin small angle scattering data  s, nm-1
ln I(s)
HOTag-PA-Ubiquitin Guinier plot ln 2.67×10-2 Rg: 3.7 nm 0 (3.7 nm)-2 s2
HOTag-PA-Ubiquitin Kratky plot 1.104 0 3 sRg
HOTag-PA-Ubiquitin pair distance distribution function Rg: 3.8 nm 0 Dmax: 12.9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin in 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH 6.8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 4.7 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 24 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

HOTag6 is a ~30-amino-acid tetramerization domain followed by a Pro-Ala linker followed by the sequence for human monoubiquitin (UniProt ID: P0CG47,, amino acids 1-76) . This is a designed protein (no natural variant exists). The HOTag6 sequence is from the Shu group at the University of California, San Francisco (

Mol. type   Protein
Organism   synthetic construct
Olig. state   Tetramer
Mon. MW   12.8 kDa
Sequence   FASTA