Decoding optimal ligand design for multicomponent condensates

Sarasi Galagedera.

SASDRB7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin

MWI(0) 53 kDa
MWexpected 53 kDa
VPorod 78 nm3
log I(s) 3.13×100 3.13×10-1 3.13×10-2 3.13×10-3
HOTag-(PA)4-Ubiquitin small angle scattering data  s, nm-1
ln I(s)
HOTag-(PA)4-Ubiquitin Guinier plot ln 3.13×100 Rg: 4.1 nm 0 (4.1 nm)-2 s2
HOTag-(PA)4-Ubiquitin Kratky plot 1.104 0 3 sRg
HOTag-(PA)4-Ubiquitin pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin in 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH 6.8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 4.5 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 24 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

HOTag6 is a ~30-amino-acid tetramerization domain followed by a (Pro-Ala)4 linker followed by the sequence for human monoubiquitin (UniProt ID: P0CG47,, amino acids 1-76) . This is a designed protein (no natural variant exists). The HOTag6 sequence is from the Shu group at the University of California, San Francisco (

Mol. type   Protein
Organism   synthetic construct
Olig. state   Tetramer
Mon. MW   13.3 kDa
Sequence   FASTA